Composite

Part:BBa_K5398605:Design

Designed by: qijia ren   Group: iGEM24_NAU-CHINA   (2024-09-10)


pET-28a(+)-Mfp6


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none.


Source

This sequence is derived from the sequence presented in the article. (doi:10.1002/btpr.1810). Besides, the Mfp6(363 bp) was cloned from the pETDuet-1-Mfp6 vector which was synthesized by the company.

References

[1] Nicklisch SC, Das S, Martinez Rodriguez NR, et al. Antioxidant efficacy and adhesion rescue by a recombinant mussel foot protein-6[J]. Biotechnol Prog, 2013, 29(6):1587-1593.

[2] TAN D, ZHAO J P, RAN G Q, et al. Highly efficient biocatalytic synthesis of L-DOPA using in situ immobilized Verrucomicrobium spinosum tyrosinase on polyhydroxyalkanoate nano-granules [J]. Appl. Microbiol. Biotechnol., 2019, 103(14): 5663-78.

[3] YAO L, WANG X, XUE R, et al. Comparative analysis of mussel foot protein 3B co-expressed with tyrosinases provides a potential adhesive biomaterial [J]. Int. J. Biol. Macromol., 2022, 195: 229-36.